ABSTRACT
[Objective] At present, a novel emerging Coronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), has caused an epidemic in the world, seriously threatening human life and health. To establish a basis for a rapid detection method for SARS-CoV-2, the monoclonal antibodies targeting nucleocapsid (N)gene were prepared and identified in this study. [Methods] BA LB/c mice were immunized with purified SARS-CoV-2 N recombinant protein. After four times of immunization. the spleen cells of the mice were fused with SP2/0 myeloma cells. The positive hybridoma cell lines stably secreting monoclonal anti-body were screened through ELISA, and the reactivity of the monoclonal antibody was further determined by western blot and indirect immunofluorescence assay. [Results] After three subclones, two hybridoma cell lines designated as 2D11 and 8G6 were obtained. and the prepared antibodies showed good reactivity with the eukaryotic expression of SARS-CoV-2 N protein. [Conclusion] The monoclonal antibody manufactured in this study can be used for SARS-CoV-2 detection.